Identification and characterization of the biotechnological potential of a wild strain of Paraconiothyrium sp.
Identifieur interne : 000747 ( Main/Exploration ); précédent : 000746; suivant : 000748Identification and characterization of the biotechnological potential of a wild strain of Paraconiothyrium sp.
Auteurs : Marina Arredondo-Santoyo [Mexique] ; Ma Soledad Vázquez-Garcidue As [Mexique] ; Gerardo Vázquez-Marrufo [Mexique]Source :
- Biotechnology progress [ 1520-6033 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : ADN ribosomique, Ascomycota, Laccase, Mycelium.
- méthodes : Biotechnologie.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : DNA, Ribosomal, Laccase.
- metabolism : Ascomycota, Mycelium.
- methods : Biotechnology.
Abstract
The isolation and characterization of fungal strains from poorly described taxa allows undercover attributes of their basic biology useful for biotechnology. Here, a wild fungal strain (CMU-196) from recently described Paraconiothyrium genus was analyzed. CMU-196 was identified as Paraconiothyrium brasiliense by phylogenetic analysis of the rDNA internal transcribed spacer region (ITS). CMU-196 metabolized 57 out of 95 substrates of the Biolog FF microplates. Efficient assimilation of dextrins and glycogen indicates that CMU-196 is a good producer of amylolytic enzymes. It showed a remarkably assimilation of α-d-lactose, substrate described as inducer of cellulolytic activity but poorly assimilated by several fungi. Metabolically active mycelium of the strain decolorized broth supplemented with direct blue 71, Chicago sky blue and remazol brilliant blue R dyes. The former two dyes were also well removed from broth by mycelium inactivated by autoclaving. Both mycelia had low efficiency for removing fuchsin acid from broth and for decolorizing wastewater from the paper industry. CMU-196 strain showed extracellular laccase activity when potato dextrose broth was supplemented with Cu+2 , reaching a maximum activity of 46.8 (±0.33) U L-1 . Studied strain antagonized phytopathogenic Colletotrichum spp. fungi and Phytophthora spp. oomycetes in vitro, but is less effective towards Fusarium spp. fungi. CMU-196 antagonism includes overgrowing the mycelia of phytopathogens and growth inhibition, probably by hydrosoluble extracellular metabolites. The biotechnological potential of strain CMU-196 here described warrants further studies to have a more detailed knowledge of the mechanisms associated with its metabolic versatility, capacity for environmental detoxification, extracellular laccase production, and antagonism against phytopathogens. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:846-857, 2018.
DOI: 10.1002/btpr.2653
PubMed: 29708651
Affiliations:
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sortKey="Vazquez Garcidue As, Ma Soledad" sort="Vazquez Garcidue As, Ma Soledad" uniqKey="Vazquez Garcidue As M" first="Ma Soledad" last="Vázquez-Garcidue As">Ma Soledad Vázquez-Garcidue As</name><affiliation wicri:level="1"><nlm:affiliation>División de Estudios de Posgrado, Facultad de Ciencias Médicas y Biológicas Dr. Ignacio Chávez, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, México.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Mexique</country><wicri:regionArea>División de Estudios de Posgrado, Facultad de Ciencias Médicas y Biológicas Dr. Ignacio Chávez, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán</wicri:regionArea><wicri:noRegion>Michoacán</wicri:noRegion></affiliation></author><author><name sortKey="Vazquez Marrufo, Gerardo" sort="Vazquez Marrufo, Gerardo" uniqKey="Vazquez Marrufo G" first="Gerardo" last="Vázquez-Marrufo">Gerardo Vázquez-Marrufo</name><affiliation wicri:level="1"><nlm:affiliation>Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, México.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Mexique</country><wicri:regionArea>Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán</wicri:regionArea><wicri:noRegion>Michoacán</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biotechnology progress</title><idno type="eISSN">1520-6033</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (metabolism)</term><term>Biotechnology (methods)</term><term>DNA, Ribosomal (metabolism)</term><term>Laccase (metabolism)</term><term>Mycelium (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN 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biology useful for biotechnology. Here, a wild fungal strain (CMU-196) from recently described Paraconiothyrium genus was analyzed. CMU-196 was identified as Paraconiothyrium brasiliense by phylogenetic analysis of the rDNA internal transcribed spacer region (ITS). CMU-196 metabolized 57 out of 95 substrates of the Biolog FF microplates. Efficient assimilation of dextrins and glycogen indicates that CMU-196 is a good producer of amylolytic enzymes. It showed a remarkably assimilation of α-d-lactose, substrate described as inducer of cellulolytic activity but poorly assimilated by several fungi. Metabolically active mycelium of the strain decolorized broth supplemented with direct blue 71, Chicago sky blue and remazol brilliant blue R dyes. The former two dyes were also well removed from broth by mycelium inactivated by autoclaving. Both mycelia had low efficiency for removing fuchsin acid from broth and for decolorizing wastewater from the paper industry. CMU-196 strain showed extracellular laccase activity when potato dextrose broth was supplemented with Cu<sup>+2</sup> , reaching a maximum activity of 46.8 (±0.33) U L<sup>-1</sup> . Studied strain antagonized phytopathogenic Colletotrichum spp. fungi and Phytophthora spp. oomycetes in vitro, but is less effective towards Fusarium spp. fungi. CMU-196 antagonism includes overgrowing the mycelia of phytopathogens and growth inhibition, probably by hydrosoluble extracellular metabolites. The biotechnological potential of strain CMU-196 here described warrants further studies to have a more detailed knowledge of the mechanisms associated with its metabolic versatility, capacity for environmental detoxification, extracellular laccase production, and antagonism against phytopathogens. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:846-857, 2018.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29708651</PMID><DateCompleted><Year>2019</Year><Month>07</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>22</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6033</ISSN><JournalIssue CitedMedium="Internet"><Volume>34</Volume><Issue>4</Issue><PubDate><Year>2018</Year><Month>07</Month></PubDate></JournalIssue><Title>Biotechnology progress</Title><ISOAbbreviation>Biotechnol Prog</ISOAbbreviation></Journal><ArticleTitle>Identification and characterization of the biotechnological potential of a wild strain of Paraconiothyrium sp.</ArticleTitle><Pagination><MedlinePgn>846-857</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/btpr.2653</ELocationID><Abstract><AbstractText>The isolation and characterization of fungal strains from poorly described taxa allows undercover attributes of their basic biology useful for biotechnology. Here, a wild fungal strain (CMU-196) from recently described Paraconiothyrium genus was analyzed. CMU-196 was identified as Paraconiothyrium brasiliense by phylogenetic analysis of the rDNA internal transcribed spacer region (ITS). CMU-196 metabolized 57 out of 95 substrates of the Biolog FF microplates. Efficient assimilation of dextrins and glycogen indicates that CMU-196 is a good producer of amylolytic enzymes. It showed a remarkably assimilation of α-d-lactose, substrate described as inducer of cellulolytic activity but poorly assimilated by several fungi. Metabolically active mycelium of the strain decolorized broth supplemented with direct blue 71, Chicago sky blue and remazol brilliant blue R dyes. The former two dyes were also well removed from broth by mycelium inactivated by autoclaving. Both mycelia had low efficiency for removing fuchsin acid from broth and for decolorizing wastewater from the paper industry. CMU-196 strain showed extracellular laccase activity when potato dextrose broth was supplemented with Cu<sup>+2</sup> , reaching a maximum activity of 46.8 (±0.33) U L<sup>-1</sup> . Studied strain antagonized phytopathogenic Colletotrichum spp. fungi and Phytophthora spp. oomycetes in vitro, but is less effective towards Fusarium spp. fungi. CMU-196 antagonism includes overgrowing the mycelia of phytopathogens and growth inhibition, probably by hydrosoluble extracellular metabolites. The biotechnological potential of strain CMU-196 here described warrants further studies to have a more detailed knowledge of the mechanisms associated with its metabolic versatility, capacity for environmental detoxification, extracellular laccase production, and antagonism against phytopathogens. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:846-857, 2018.</AbstractText><CopyrightInformation>© 2018 American Institute of Chemical Engineers.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Arredondo-Santoyo</LastName><ForeName>Marina</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, México.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vázquez-Garcidueñas</LastName><ForeName>Ma Soledad</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>División de Estudios de Posgrado, Facultad de Ciencias Médicas y Biológicas Dr. Ignacio Chávez, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, México.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vázquez-Marrufo</LastName><ForeName>Gerardo</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0003-4683-699X</Identifier><AffiliationInfo><Affiliation>Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, México.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>07</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Prog</MedlineTA><NlmUniqueID>8506292</NlmUniqueID><ISSNLinking>1520-6033</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004275">DNA, Ribosomal</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.2</RegistryNumber><NameOfSubstance UI="D042845">Laccase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001203" MajorTopicYN="N">Ascomycota</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004275" MajorTopicYN="N">DNA, Ribosomal</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042845" MajorTopicYN="N">Laccase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025282" MajorTopicYN="N">Mycelium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Paraconiothyrium brasiliense</Keyword><Keyword MajorTopicYN="Y">antagonism</Keyword><Keyword MajorTopicYN="Y">bioremediation</Keyword><Keyword MajorTopicYN="Y">laccase</Keyword><Keyword MajorTopicYN="Y">metabolism</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>07</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>04</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>5</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>7</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>5</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29708651</ArticleId><ArticleId IdType="doi">10.1002/btpr.2653</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Mexique</li></country></list><tree><country name="Mexique"><noRegion><name sortKey="Arredondo Santoyo, Marina" sort="Arredondo Santoyo, Marina" uniqKey="Arredondo Santoyo M" first="Marina" last="Arredondo-Santoyo">Marina Arredondo-Santoyo</name></noRegion><name sortKey="Vazquez Garcidue As, Ma Soledad" sort="Vazquez Garcidue As, Ma Soledad" uniqKey="Vazquez Garcidue As M" first="Ma Soledad" last="Vázquez-Garcidue As">Ma Soledad Vázquez-Garcidue As</name><name sortKey="Vazquez Marrufo, Gerardo" sort="Vazquez Marrufo, Gerardo" uniqKey="Vazquez Marrufo G" first="Gerardo" last="Vázquez-Marrufo">Gerardo Vázquez-Marrufo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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